_x000d_
_x000d_
Source protein:
_x000d_
_x000d_
Protein A affinity_x000d_
chromatography purified protein from mouse ascites fluid.
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_x000d_
_x000d_
Clonality and Host Species:
_x000d_
_x000d_
Myc-A7, Mouse
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_x000d_
_x000d_
Immunogen and Isotype:
_x000d_
_x000d_
EQKLISEEDL (Myc) synthetic_x000d_
peptide conjugated to KLH, IgG1
_x000d_
_x000d_
_x000d_
Specificity and reactivity:
_x000d_
_x000d_
Detects the N-terminal_x000d_
or C-terminal Myc -tagged fusion proteins.
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_x000d_
_x000d_
_x000d_
Application:
_x000d_
_x000d_
Western blot, Dot blot,_x000d_
ELISA, IHC/IS, Western blot (with ECL) 10-50 ng of Myc-tagged fusion protein.
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_x000d_
_x000d_
Available form:
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_x000d_
Lyophilized 1mg/mL (lyophilized_x000d_
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
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_x000d_
_x000d_
Working procedure:
_x000d_
_x000d_
Western blot (with ECL)_x000d_
1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best_x000d_
results, determine the optimal working antibody dilution for your protein of_x000d_
interest.
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_x000d_
_x000d_
Storage:
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_x000d_
Supplied lyophilized_x000d_
antibodies can be stored at 200C for 3 years.
_x000d_
_x000d_
Reconstitute the_x000d_
lyophilized antibody with distilled water to 1mg/ml or a desired concentration_x000d_
before use.
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_x000d_
Reconstituted liquid can_x000d_
be stored at -20oC for 2 years. Centrifuge after first thaw to_x000d_
maximum product recovery. Aliquot into smaller volume to avoid repeated_x000d_
freeze-thaw cycles. Store aliquots at 40C_x000d_
for several days to 2 weeks.