General Description
Source protein: Protein A affinity chromatography purified protein from mouse ascites fluid
Clonality and Host Species: V5.E10, Mouse
Immunogen and Isotype: GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH, IgG2a
Specificity and reactivity: Detects V5 -tagged fusion proteins.
Application: Western blot, Dot blot, ELISA, IHC/IS. Western blot (with ECL) 1-5 ng of V5-tagged fusion protein.
Available form: 50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Working procedure: Western blot (with ECL) 1:1000-3,000 dilution, incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.
Storage: Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4ºC for several days to 2 weeks.